| Abstract |
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L-isoleucine production by ethanol utilizing micro-organism. |
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| 270 micro-organisms isolated from soil by enrichment culture and able to
grow on ethanol as the sole source of carbon and energy were screened for
their ability to produce L-isoleucine. A process using a mutant of Brevibacterium flavum resistant to DL-alpha-amino butyrate (alpha-AB) was optimised using ethanol as subsrate and alpha-AB as precursor. This organism did not produce significant quantities of L-leucine or L-valine, thus simplifying L-isoleucine recovery. |