The phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) catalyzes carbohydrate transport by coupling it to phosphorylation. Previously, we reported a Corynebacterium glutamicum R beta-glucoside PTS encoded by bglF. Here we report that C. glutamicum R encodes an additional beta-glucoside PTS gene, bglF2, organized in a cluster with putative phospho-beta-glucosidase, bglA2, and putative antiterminator, bglG2. While single gene disruption strains of either bglF or bglF2 was able to utilize salicin or arbutin as sole carbon sources, a double disruption strain exhibited defects in utilization of both carbon sources. Expression of both bglF and bglF2 was induced in the presence of either salicin or arbutin, though disruption of bglG2 affected only bglF2 expression. Moreover, in the presence of glucose and either salicin or arbutin, glucose completely repressed the expression of bglF but hardly that of bglF2. We conclude that BglF and BglF2 have a redundant role in beta-glucoside transport even as catabolite repression control of their encoding genes is different. We also show that expression of both bglF and bglF2 genes requires general PTS.